Plant tissue culture, also known as in vitro culture or micropropagation, is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. The Principle of Totipotency
Overcoming seed dormancy or rescuing hybrid embryos from incompatible crosses. 6. PPT Slide Outline Template plant tissue culture ppt pdf
The explant is treated with chemical sterilants (e.g., sodium hypochlorite, ethanol, or mercuric chloride) to kill surface microbes without damaging the plant tissue. Plant tissue culture, also known as in vitro
Poor sterilization, weak aseptic technique, fungal spores, or bacterial endophytes. PPT Slide Outline Template The explant is treated
A gelled (agar) or liquid nutrient medium containing:
The entire field of tissue culture rests on the concept of . This is the biological ability of a single plant cell to regenerate into a whole, multicellular plant. Unlike animal cells, which become highly specialized and lose flexibility, many differentiated plant cells retain the genetic "blueprint" to restart the entire growth process. 2. Core Techniques in Tissue Culture
Successfully cultured tomato roots continuously, demonstrating the feasibility of organ culture.